Image Registration Scenario

Warning

This section is under construction! Some information might be missing.

Perform iterative registration within one registration workspace

1. Browsing

   • Load image CZI/qDII-CLV3-PIN1-PI-E37-LD-SAM7-T0-P3.czi

   • Export to manager

   • Load image CZI/qDII-CLV3-PIN1-PI-E37-LD-SAM7-T10-P3.czi

   • Export to manager

2. Registration The layout of the workspace registration is as follow:

Input	Output
floating image	stack + push button
reference image	output image + export button

• top view (floating) -> TO

• bottom view (reference) -> T10

• algo registrationTimagetk

  • Method -> rigid

  • Channel -> Ch1_EBFP

  • Apply -> It fills the output view (bottom right)

• Sync output and reference view to compare

• Press “Iterate ⇧” button to validate results

  • output integrates the stack (top right)

  • transformation and image are stored

  • stack elements are highlighted

  • output image moves to floating view (with an animation)

  • output view is cleared

• algo registrationTimagetk

  • Method -> affine

  • Apply

    • Last image from the stack is passed as input as it was previously moved into the floating image view

    • Computes registration -> incremental transformation

    • Fills the output view with registered image

  • Press “Push ⇧” button to validate results

  • Come down one level in the stack

    • anterior image moves to the input view

    • output view is cleared

  • Change parameter values and press apply

  • Press “Push ⇧” button to validate results

    • posterior stack items are deleted (with an animation)

    • new output integrates the stack

  • Press Export to save the actual image into the world and add a new node in the pipeline. When exporting the pipeline, we also save the stack composition to be able to redo, re-load the computation.

Video